Journal: Aging (Albany NY)

Article Title: Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation

doi: 10.18632/aging.203804

Figure Lengend Snippet: Primer sequences and product length.

Article Snippet: Commercial ELISA kits were used to measure CEA, CK18, CK20, MUC13, CK8 and EPCAM (CUSABIO, China).

Techniques: Sequencing, Amplification, Real-time Polymerase Chain Reaction, Methylation

Journal: Aging (Albany NY)

Article Title: Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation

doi: 10.18632/aging.203804

Figure Lengend Snippet: The serum levels of CEA, CK18, CK20, CK8, MUC13 and EPCAM in CRC, CA patients and healthy controls. ( A ) Screening specific genes that encode secretory proteins in CRC by bioinformatics. ( B ) Testing the expression of candidate serum biomarker genes by Q-PCR in 8 cell lines. ( C ) Detection of the serum levels of CEA, CK18, CK20, CK8, MUC13 and EPCAM in CRC, CA patients and healthy controls by ELISA.

Article Snippet: Commercial ELISA kits were used to measure CEA, CK18, CK20, MUC13, CK8 and EPCAM (CUSABIO, China).

Techniques: Expressing, Biomarker Discovery, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Identification of the best housekeeping gene for RT-qPCR analysis of human pancreatic organoids

doi: 10.1371/journal.pone.0260902

Figure Lengend Snippet: The relative transcriptional level of the two ductal markers EpCAM and SOX9 in each sample condition considered was normalized with the best and the worst reference gene (RPL13A and ACTB, respectively), the geometric mean (Geo.mean) of two selected HKGs by NormFinder (PPIA and UBC) and one commonly used gene (GAPDH). One-way analysis of variance followed by Dunnett’s post-hoc test for multiple comparisons was used.

Article Snippet: We stained the samples using 1:200 FITC mouse Anti-Human Epithelial Cell Adhesion Molecule (EpCAM; BD, cat. #347197), 1:100 Alexa Fluor® 647 Mouse Anti- SRY-box transcription factor 9 (Sox9; BD, cat. #565493) and 1:2000 FITC-conjugated lectin from Ulex europaeus (UEA1) (Sigma-Aldrich, cat. #L9006).

Techniques: